Genetic analysis of self-associating immunoglobulin G rheumatoid factors from two rheumatoid synovia implicates an antigen-driven response.

Although much has been learned about the molecular basis of immunoglobulin M (IgM) rheumatoid factors (RFs) in healthy individuals and in patients with mixed cryoglobulinemia and rheumatoid arthritis, little is known about the genetic origins of the potentially pathogenic IgG RFs in the inflamed rheumatoid synovia of patients. Recently, we generated from unmanipulated synovium B cells several hybridomas that secreted self-associating IgG RFs. To delineate the genetic origins of such potentially pathogenic RFs, we adapted the anchored polymerase chain reaction to rapidly clone and characterize the expressed Ig V genes for the L1 and the D1 IgG RFs. Then, we identified the germline counterparts of the expressed L1 IgG RF V genes. The results showed that the L1 heavy chain was encoded by a Vh gene that is expressed preferentially during early ontogenic development, and that is probably located within 240 kb upstream of the Jh locus. The overlap between this RF Vh gene and the restricted fetal antibody repertoire is reminiscent of the natural antibody-associated Vh genes, and suggests that at least part of the "potential pathogenic" IgG RFs in rheumatoid synovium may derive from the "physiological" natural antibody repertoire in a normal immune system. Indeed, the corresponding germline Vh gene for L1 encodes the heavy chain of an IgM RF found in a 19-wk-old fetal spleen. Furthermore, the comparisons of the expressed RF V genes and their germline counterparts reveal that the L1 heavy and light chain variable regions had, respectively, 16 and 7 somatic mutations, which resulted in eight and four amino acid changes. Strikingly, all eight mutations in the complementarity determining regions of the V gene-encoded regions were replacement changes, while only 6 of 11 mutations in the framework regions caused amino acid changes. Combined with L1's high binding affinity toward the Fc fragment, these results suggest strongly that the L1 IgG RF must have been driven by the Fc antigen

The Necessary and Sufficient Conditions of Separability for Multipartite Pure States

In this paper we present the necessary and sufficient conditions of separability for multipartite pure states. These conditions are very simple, and they don't require Schmidt decomposition or tracing out operations. We also give a necessary condition for a local unitary equivalence class for a bipartite system in terms of the determinant of the matrix of amplitudes and explore a variance as a measure of entanglement for multipartite pure states.Comment: Submitted to PRL in Sep. 2004, the paper No is LV9637. Submitted to SIAM on computing, in Jan., 2005, the paper No. is SICOMP 44687. Under reviewing no

Robotic surgery for gynecologic cancers: indications, techniques and controversies.

Minimally invasive surgery for gynecologic cancers is associated with fewer postoperative complications including less blood loss and quicker recovery time compared to traditional laparotomy. The robotic platform has allowed patients access to minimally invasive surgery due to its increased utilization by gynecologic oncologists. Many surgeons have embraced the robotic platform due to its technological advances over traditional laparoscopy including high-definition 3D optics, wristed instrumentation, camera stability and improved ergonomics. While robotic surgery continues as a mainstay in the management of gynecologic cancers, it remains controversial in regards to its cost effectiveness and more recently, its long-term impact on clinical and oncologic outcomes. A strong component of the justification of this surgical platform is based on extrapolated data from traditional laparoscopy despite limited prospective randomized trials for robotic-assisted surgery. In this review, we highlight the use of robotic surgery in the management of gynecologic cancers in special populations: fertility sparing patients, the morbidly obese, the elderly, and patients with a favorable response to neoadjuvant chemotherapy

Design Principles for High-Capacity Mn-Based Cation-Disordered Rocksalt Cathodes

Mn-based Li-excess cation-disordered rocksalt (DRX) oxyfluorides are promising candidates for next-generation rechargeable battery cathodes owing to their large energy densities, the earth abundance, and low cost of Mn. In this work, we synthesized and electrochemically tested four representative compositions in the Li-Mn-O-F DRX chemical space with various Li and F content. While all compositions achieve higher than 200 mAh g−1 initial capacity and good cyclability, we show that the Li-site distribution plays a more important role than the metal-redox capacity in determining the initial capacity, whereas the metal-redox capacity is more closely related to the cyclability of the materials. We apply these insights and generate a capacity map of the Li-Mn-O-F chemical space, LixMn2-xO2-yFy (1.167 ≤ x ≤ 1.333, 0 ≤ y ≤ 0.667), which predicts both accessible Li capacity and Mn-redox capacity. This map allows the design of compounds that balance high capacity with good cyclability

The Moment of Inertia and the Scissors Mode of a Bose-condensed Gas

We relate the frequency of the scissors mode to the moment of inertia of a trapped Bose gas at finite temperature in a semi-classical approximation. We apply these theoretical results to the data obtained in our previous study of the properties of the scissors mode of a trapped Bose-Einstein condensate of $^{87}$Rb atoms as a function of the temperature. The frequency shifts that we measured show quenching of the moment of inertia of the Bose gas at temperatures below the transition temperature - the system has a lower moment of inertia that of a rigid body with the same mass distribution, because of superfluidity.Comment: 14 pages, 5 fig

Static Observers in Curved Spaces and Non-inertial Frames in Minkowski Spacetime

Static observers in curved spacetimes may interpret their proper acceleration as the opposite of a local gravitational field (in the Newtonian sense). Based on this interpretation and motivated by the equivalence principle, we are led to investigate congruences of timelike curves in Minkowski spacetime whose acceleration field coincides with the acceleration field of static observers of curved spaces. The congruences give rise to non-inertial frames that are examined. Specifically we find, based on the locality principle, the embedding of simultaneity hypersurfaces adapted to the non-inertial frame in an explicit form for arbitrary acceleration fields. We also determine, from the Einstein equations, a covariant field equation that regulates the behavior of the proper acceleration of static observers in curved spacetimes. It corresponds to an exact relativistic version of the Newtonian gravitational field equation. In the specific case in which the level surfaces of the norm of the acceleration field of the static observers are maximally symmetric two-dimensional spaces, the energy-momentum tensor of the source is analyzed.Comment: 28 pages, 4 figures

Pion Form Factors in Holographic QCD

Using a holographic dual model of QCD, we compute the pion electromagnetic form factor F_pi(Q^2) in the spacelike momentum transfer region, as well as pion couplings to vector mesons g_rho^(n) pi pi. Spontaneous and explicit chiral symmetry breaking are intrinsic features of this particular holographic model. We consider variants with both ``hard-wall'' and ``soft-wall'' infrared cutoffs, and find that the F_pi(Q^2) data tend to lie closer to the hard-wall model predictions, although both are too shallow for large Q^2. By allowing the parameters of the soft-wall model (originally fixed by observables such as m_rho) to vary, one finds fits that tend to agree better with F_pi(Q^2). We also compute the pion charge radius for a variety of parameter choices, and use the values of f^(n)_rho, g_{rho^(n) pi pi} and m^(n)_rho to observe the saturation of F_pi(0) by rho poles.Comment: 17 pages, 2 figures, revised fits using consistent normalization of f_pi. References update